my $input_dir = "/Users/logancurtis-whitchurch/Desktop/IB_Senior_Thesis/consensus_files/mask_files/"; #directory with mask files my $input_file = "$input_dir"."mask."."$population".".txt"; open(CGS, "<$input_file") or die "can't open input_file\n"; my $cgs = ; my $interval = "/Users/logancurtis-whitchurch/Desktop/chrX_divisions/"."$region"."_$filter".".txt"; #specifiecs intervals by region and filter version open (INTERVAL, "<$interval") or die "can't open interval file\n"; foreach ( ) { my (undef, $start, $end) = split '\s+', $_; my $subs_length = $end-$start; my $included_length = substr( $cgs, $start, $subs_length ); print length $included_length; printf "found %d zeros\n", $included_length =~ tr[0][]; }