#!/usr/bin/perl use 5.014; # 986041 my $str="BATCATDATEFEAT"; my $output = 'Results.txt'; open(my $fh, '>', $output) or die "Can't open $output, $!"; my %spots; while ($str =~ /([AT])/ig) { push @{$spots{uc $1}}, pos $str; } for my $key (keys %spots) { my $i = 0; for my $pos (@{$spots{$key}}) { $i++; my $text = "\n $key=$i ends at $pos\n"; print $text; print $fh $text; } } =head execution: C:\>type Results.txt A=1 ends at 2 A=2 ends at 5 A=3 ends at 8 A=4 ends at 13 T=1 ends at 3 T=2 ends at 6 T=3 ends at 9 T=4 ends at 14 =cut