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in reply to Re^2: Iso electric point calculation using perl
in thread Iso electric point calculation using perl

The command at the console was perl your_prog_name.pl fasta_input_file.fasta. I don't know what lines 104 and 107 are. Please put them here.

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Re^4: Iso electric point calculation using perl
by yuvraj_ghaly (Sexton) on Jul 23, 2013 at 04:19 UTC

    line 104

          $result += $acid{$aa}/(1+10**($pka{$aa}-$pI));

    line 107

          $result -= $base{$aa}/(1+10**($pI-$pka{$aa}));

      You must have forgotten to declare those variables. If you run it without using strict it won't raise an error.

        thank you