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subhomoi.bk has asked for the wisdom of the Perl Monks concerning the following question:

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Re: siRna prediction
by moritz (Cardinal) on May 20, 2011 at 09:25 UTC

      and with rounded corners and a padded top. Didn't you take notes in the CB?

      True laziness is hard work

      Bah, everyone knows mauve has more RAM.

      And it should have flames down the side. And fuzzy dice. And an attachment to julienne fries.

      And a pony.

      The cake is a lie.
      The cake is a lie.
      The cake is a lie.

Re: siRna prediction
by marto (Cardinal) on May 20, 2011 at 09:17 UTC

      Thanks... but i m new to bioperl..plz help me on this..when i used the following code...an error msg comes "Target sequence not defined"..so where is $bio_seq?

      use Bio::Tools::SiRNA; my $sirna_designer = Bio::Tools::SiRNA->new( -target => $bio_seq, -rules => 'tuschl' ); my @pairs = $sirna_designer->design; foreach $pair (@pairs) { my $sense_oligo_sequence = $pair->sense->seq; my $antisense_oligo_sequence = $pair->antisense->seq; # print out results print join ("\t", $pair->start, $pair->end, $pair->rank, $sense_oligo_sequence, $antisense_oligo_sequence), " +\n"; }
Re: siRna prediction
by ww (Archbishop) on May 20, 2011 at 11:55 UTC
    I'd guess that it'll be more marketable if the double helix pattern is a prominent part of the decoration (perhaps in gold, to contrast with the blue base recommended above), and that all graphic ads use a viewpoint that emphasize the way the tool's shape itself suggests a double helix.