http://www.perlmonks.org?node_id=1045582


in reply to Re: Iso electric point calculation using perl
in thread Iso electric point calculation using perl

thank you for the code but I have one thing to ask. I should incorporate this code into my perl script. m I right?

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Re^3: Iso electric point calculation using perl
by erix (Prior) on Jul 22, 2013 at 05:19 UTC

    No. I didn't look at your code. It's obviously too long and you said it didn't do what you wanted and I was only interested in the code on the rutgers.edu website so I made a program to wrap that into.

    Just take the code I gave you, save it as a file, and copy the code from the rutgers.edu website into the calculate_protein_details sub that I gave you (except the first line (of the rutgers.edu code)). Then it just works. I tried it again, just now. If you want it to read the sequence from a fasta file, add that functionality afterwards, but that's a completely different 'problem'.

    Remember: when things don't work you have to simplify till you understand why it doesn't.