Hi! Thank you so much for your reply! So the goal I try to achieve here is that as for the 21mer I'm working on, it is a sequence of 21 letters, each position can have 4 variable "AGCT". Therefore, in total, it should have 4**21 combination. I have total 197 kmer files, each of them contains around 8000 of these 21mers, and I'm trying to count how many time each 21mers has shown among these 197 files. That's why I need a matrix to do so.
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