First off, thanks for all the replies guys! I shouldn't have rushed my posting since I left out some much needed information so sorry about that.
Here is a sample entry in the genbank file. The biotype entry is after "/note". I thought I would have to do all lower case because a couple of the entries weren't uniform and had uppercase letters; is this not needed?
LOCUS EU099432 832 bp DNA linear INV 27
+-APR-2009
DEFINITION Bemisia tabaci strain 05-06 cytochrome oxidase subunit I-l
+ike (COI)
gene, partial sequence; mitochondrial.
ACCESSION EU099432
VERSION EU099432.1 GI:158726330
KEYWORDS .
SOURCE mitochondrion Bemisia tabaci
ORGANISM Bemisia tabaci
Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygo
+ta;
Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aleyrod
+iformes;
Aleyrodoidea; Aleyrodidae; Aleyrodinae; Bemisia.
REFERENCE 1 (bases 1 to 832)
AUTHORS Ma,W.H., Li,X.C., Dennehy,T.J., Lei,C.L., Wang,M., Degain,
+B.A. and
Nichols,R.L.
TITLE Utility of MtCOI polymerase chain reaction-restriction fra
+gment
length polymorphism in differentiating between Q and B whi
+tefly
Bemisia tabaci biotypes
JOURNAL Insect Sci. 16 (2), 107-114 (2009)
REFERENCE 2 (bases 1 to 832)
AUTHORS Ma,W., Li,X., Degain,B. and Dennehy,T.
TITLE Direct Submission
JOURNAL Submitted (13-AUG-2007) Entomology, The University of Ariz
+ona, 1140
E. 4th St., Tucson, AZ 85721, USA
FEATURES Location/Qualifiers
source 1..832
/organism="Bemisia tabaci"
/organelle="mitochondrion"
/mol_type="genomic DNA"
/strain="05-06"
/db_xref="taxon:7038"
/country="USA: Arizona"
/note="biotype: B"
gene <1..>832
/gene="COI"
misc_feature <1..>832
/gene="COI"
/note="similar to cytochrome oxidase subunit I"
ORIGIN
1 atatgcatgg agtgattttt tggtccccca gaagtaatat ggcagattag tgcat
+tggac
61 ttgatttgtt tggtcatcca taaggcaaaa ggcacaatag ggcttcgaag gttta
+ttgtt
121 tgacgtcctc atatattcac agttggaata gatgtagata ctcgagctta tttca
+cttca
181 gccactataa ttattgctgt tcccacagga attaaaattt ttagttggct tgcta
+ctttg
241 ggtggaataa agtctaataa attaaggcct cttggccttt gatttacagg atttt
+tattt
301 ttatttacta taggtgggtt aactggaatt attcttggta attcttctgt agatg
+tgtgt
361 ctgcatgaca cttattttgt tgttgcacat tttcattatg ttttatcaat aggaa
+ttatt
421 tttgctattg taggaggagt tatctattga tttccactaa tcttaggttt aacct
+taaat
481 aattatagat tggtgtctca attttatatc atgtttattg gagtaaattt aactt
+ttttt
541 cctcaccatt ttcttggttt agggggaatg cctcgtcgat attcagatta tgctg
+attgc
601 tatctagtat gaaataaaat ttcttctgcg ggaaggattc tgagtattat ttctg
+ttatt
661 tattttttat ttattgtttt agaatccttt cttcttctgc ggttagtaag attta
+agctt
721 ggtgtaagta ggcatctaga atgaaagatt aataaaccag ctcttaatca cagtt
+ttaaa
781 gagttgtgtt taactttttt tttctaatat ggcagattag ggccccggga aa
//
Here is my updated code that now contains more errors lol.
# Genbank Splitter
# Takes Accession number and biotype as name for new FASTA file
# Contents of new FASTA file is corresponding sequence
use strict;
use warnings;
$/ = "//";
# Constants
my $genfile = 'c:\bemisia_coi.gb';
my $outfile = "$accession_$biotype";
my ($OUT, $IN);
print "Input: $genfile\n";
open my $ifh, "<", $genfile or die "cannot open $genfile: $!\n";
while (my $chunk = <$ifh>){
last if eof $ifh;
$chunk = lc $chunk;
my ($accession) = $chunk =~ /locus\s*([a-z]{8});
my ($biotype) = $chunk =~ /biotype: ([a-z]{1});
my ($sequence) = $chunk =~ "/origin(\*+)\/\/\";
$sequence =~ s/\s|\d//g;
my $outfile = "${accession}_${biotype}";
open my $ofh, '>' $outfile or die "cannot open $outfile: $!\n";
print "Printing to $outfile\n";
print $ofh, ">$accession $biotype\n^^\n$sequence";
close $ofh;
}
And here are the errors I get
C:\Users\Owner>perl c:\gen_split2.pl
Bareword found where operator expected at c:\gen_split2.pl line 22, ne
+ar "my ($b
iotype) = $chunk =~ /biotype"
(Might be a runaway multi-line // string starting on line 21)
(Do you need to predeclare my?)
Backslash found where operator expected at c:\gen_split2.pl line 22, n
+ear "bioty
pe\"
Unrecognized escape \s passed through at c:\gen_split2.pl line 23.
Unrecognized escape \d passed through at c:\gen_split2.pl line 23.
Scalar found where operator expected at c:\gen_split2.pl line 25, near
+ "my $outf
ile = "${accession}"
(Might be a runaway multi-line "" string starting on line 23)
(Do you need to predeclare my?)
Bareword found where operator expected at c:\gen_split2.pl line 25, ne
+ar "${acce
ssion}_"
(Missing operator before _?)
String found where operator expected at c:\gen_split2.pl line 27, near
+ "open my
$ofh, '>' $outfile or die ""
(Might be a runaway multi-line "" string starting on line 25)
(Missing semicolon on previous line?)
Backslash found where operator expected at c:\gen_split2.pl line 27, n
+ear "$!\"
(Missing operator before \?)
String found where operator expected at c:\gen_split2.pl line 29, near
+ "print ""
(Might be a runaway multi-line "" string starting on line 27)
(Missing semicolon on previous line?)
Bareword found where operator expected at c:\gen_split2.pl line 29, ne
+ar "print
"Printing"
(Do you need to predeclare print?)
Backslash found where operator expected at c:\gen_split2.pl line 29, n
+ear "$outf
ile\"
(Missing operator before \?)
String found where operator expected at c:\gen_split2.pl line 31, near
+ "print $o
fh, ""
(Might be a runaway multi-line "" string starting on line 29)
(Missing semicolon on previous line?)
Scalar found where operator expected at c:\gen_split2.pl line 31, near
+ "$accessi
on $biotype"
Global symbol "$accession_" requires explicit package name at c:\gen_s
+plit2.pl l
ine 12.
Global symbol "$biotype" requires explicit package name at c:\gen_spli
+t2.pl line
12.
Global symbol "$biotype" requires explicit package name at c:\gen_spli
+t2.pl line
21.
syntax error at c:\gen_split2.pl line 22, near "my ($biotype) = $chunk
+ =~ /bioty
pe"
Global symbol "$chunk" requires explicit package name at c:\gen_split2
+.pl line 2
3.
Global symbol "$sequence" requires explicit package name at c:\gen_spl
+it2.pl lin
e 23.
syntax error at c:\gen_split2.pl line 25, near "my $outfile = "${acces
+sion}"
Global symbol "$accession" requires explicit package name at c:\gen_sp
+lit2.pl li
ne 25.
Global symbol "$biotype" requires explicit package name at c:\gen_spli
+t2.pl line
25.
Global symbol "$accession" requires explicit package name at c:\gen_sp
+lit2.pl li
ne 31.
c:\gen_split2.pl has too many errors.