See How do I post a question effectively?, does the module Bio::Tools::SiRNA help with your task?

Thanks... but i m new to bioperl..plz help me on this..when i used the following code...an error msg comes "Target sequence not defined"..so where is $bio_seq?

use Bio::Tools::SiRNA;

     

  my $sirna_designer = Bio::Tools::SiRNA->new( -target => $bio_seq,
                                               -rules  => 'tuschl'
    );


  my @pairs = $sirna_designer->design;


  foreach $pair (@pairs)

 {
      my $sense_oligo_sequence = $pair->sense->seq;
      my $antisense_oligo_sequence = $pair->antisense->seq;

      # print out results
      print join ("\t", $pair->start, $pair->end, $pair->rank,
                  $sense_oligo_sequence, $antisense_oligo_sequence), "
+\n";
  }
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and with rounded corners and a padded top. Didn't you take notes in the CB?

True laziness is hard work

Bah, everyone knows mauve has more RAM.

And it should have flames down the side. And fuzzy dice. And an attachment to julienne fries.

And a pony.

The cake is a lie.
The cake is a lie.
The cake is a lie.