Thank you frozenjoy. With FastQ on galaxy I need to trim the first three letters for my record to be able use the barcode splitting function. I haven't tried the stand-alone version yet. I will give that a go once I can get it to work on my computer.
These three letters are important for my analysis, so I am not entirely sure if I can use FastX's barcode splitter tool. I am playing around with the galaxy version of it at present.
in reply to Re: Deconvolutinng FastQ files
in thread Deconvolutinng FastQ files