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in reply to How can I define the subroutine to get the result of the appended string i.e. ATGCTTTT?

You need to export the DNAappend symbol. I would use DNAlibrary or some such name, presuming that you will add other DNA routines to this thing.
#!/usr/bin/perl -w use strict; use DNAlibrary; my $a='ATGC'; my $b='TTTT'; print"\n\n"; print DNAappend($a,$b), "\n\n"; #ATGCTTTT
#!/usr/bin/perl -w use strict; package DNAlibrary; #FILE name should be DNAlibrary.pm use vars qw(@ISA @EXPORT @EXPORT_OK %EXPORT_TAGS $VERSION); use Exporter; our $VERSION=1.0; our @ISA = qw(Exporter); our @EXPORT = qw(DNAappend);#these are the default exports our @EXPORT_OK = qw(); #these are export on request sub DNAappend { my ($head,$tail)=@_; return ($head.$tail) } 1;