grep -v -F -f file2 file1
English translation: using the Unix grep command, search file1 for lines which do not match ( -v ) the fixed strings ( -F ) ( as opposed to regular expressions ) found in file2 ( -f file2 ).
There are versions of grep available for Windows. Mac OS has a Unix basis, so it already has it.
If you absolutely have to do it in Perl, I would use the lines from file2 as the keys of a hash, assigning the number 1 as a value. Then, as I read the other file, it's trivial to check whether it is present in the hash.
As Occam said: Entia non sunt multiplicanda praeter necessitatem.
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