Nice example++. Thanks for posting the code.
The 2bit format is used by the BLAT program for aligning DNA sequences. The BLAT suite of programs contains two utilities that might be of interest:
- faToTwoBit – convert Fasta format sequence files to a dense randomly-accessable .2bit format
- twoBitToFa – convert from the .2bit format back to fasta
The twoBitToFa documentation is as follows:
twoBitToFa - Convert all or part of .2bit file to fasta
twoBitToFa input.2bit output.fa
-seq=name - restrict this to just one sequence
-start=X - start at given position in sequence (zero-based)
-end=X - end at given position in sequence (non-inclusive)
Once the sequence is converted back to fasta format it can be easily manipulated using a variety of tools (Perl, BioPerl, sequence utility programs, etc).
So yes, a utility does exist that meets at least some of your listed requirements. OTOH, another tool in the toolbox, especially if it is pure Perl and/or a snazzy wrapper around a command-line utility, is always welcome. :-)
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