I'd say that normally (99% of the time) I write the subject line first before writing the body. I consider email the equivalent of writing a memo or (I'm about to date myself) a paper letter. Usually when I write a letter to someone (like a vendor or client) I have a subject line right up there with the address portion of the letter such as:
Shark River Technical Solutions
123 Mystreet St.
City, State 00000-0000
September 10, 2012
321 TheirStreet St.
City, State 11111-1234
Subject: Account 123456-07 in arrears
And in fact I have my own templates for LibreOffice already built for both personal letters and business letters that plug the information in for me (since I'm lazy.)
I have, however, been known to go back and change the subject line based on the content of an email if the direction of that email changed since I first thought of writing it.
I've also been known to change a subject line for a reply to an email. For instance someone has written me an email with the subject line "Hi There!" I'll change the subject line to something like "RE: Dinner plans for Saturday (was RE: Hi There!)" so that looking at the thread later on makes sense to me.
Peter L. Berghold -- Unix Professional
Peter -at- Berghold -dot- Net; AOL IM redcowdawg Yahoo IM: blue_cowdawg
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