My goodness ... bigger type-fonts, too?
Unforrtunately, the OP really doesn’t give a terrible amount of detail .. perhaps more detail will follow? Nevertheless, what is said about the program is fairly typical: that it is a GUI program which is described as “a launching script.” (Thus my first guess: that a non-GUI implementation of the same process would behave similarly. I would not spend any time prowling around the guts of Tk looking for any performance improvement.) Likewise, the program is further described as “using sockets for IPC” and “using MySQL to store data,” both of which could just-as-equally describe roughly a million other production programs on this planet ... nearly all of which (is this one the exception?) are I/O-bound and prone to have logic-holes in how they handle sockets. It would be a rare bird indeed if, being such a program, it were throttled by nanoseconds. (And I know perfectly well that you have written just such “birds.”) Indeed, one could spend many hours “profiling” such a program, chasing nanoseconds, and although one might come up with interesting results, would never hit upon what made such a program “slow.” And that is why I suggested that this could be the wrong tree; not some secret desire to be (let alone to be called ...) “a moron” in a public place.
Do I know? No. No more than you do. Maybe the OP will elaborate further.