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Could you provide perhaps a single line or maybe even 2 or 3 of the input file?

I modified your code a bit for brevity(untested). I am not all that familiar with microbiology(been 35 years since high school biology). I suspect there are CPAN modules that will handle problems like this. Do a search for like this one at bio:: and see if there might be something you can use if that is an option for you.

Hope that is in some way helpful...

#!/usr/bin/perl use strict; use warnings; print "Select the region where you want to find CpG Island\n"; my ($a,$t,$c,$g,$cg,$total); print "1. Region 1-200\n"; print "2. Region 2-202\n"; print "3. Region 3-203\n"; my $choice =<STDIN>; chomp($choice); my $file = 'd:\perl\1.txt'; if ( $choice == 1 ) { region(0); } elsif ( $choice == 2 ) { region(1); } elsif ( $choice == 3) { region(2); } sub region { my $start = shift; open(INPUT, $file) or die ("File not found"); foreach(my $line = <INPUT>) { my $a = substr($line,$start,200) =~ tr/A//; my $t = substr($line,$start,200) =~ tr/T//; my $g = substr($line,$start,200) =~ tr/G//; my $c = substr($line,$start,200) =~ tr/C//; my $cg = substr($line,$start,200) =~ tr/ CG //; my $total = substr($line,$start,200) =~ tr/ATGC//; } } my $CpG = ($cg)/$total*100; print"Count of A = $a\n"; print"Count of T = $t\n"; print"Count of G = $g\n"; print"Count of C = $c\n"; print"CG = $cg\n"; print"total count of ATGC = $total\n"; print"CpG percentage = $CpG\n"; if ($CpG>=60) { print"CpG present"; } else { print"CpG absent"; }
Update: moved printing back outside the sub. What I get for messing with code at this hour of the morning...
...the majority is always wrong, and always the last to know about it...
Insanity: Doing the same thing over and over again and expecting different results...

In reply to Re^15: how to access elements in perl by wjw
in thread how to access elements in perl by grewal7634

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