http://www.perlmonks.org?node_id=1017336

MVRS has asked for the wisdom of the Perl Monks concerning the following question:

i tried running bioperl script to find hydropathicity of a protein sequence , and am unable to fix the error >

Error : Can't use string ("Bio::Tools::SeqStats") as a HASH ref while "strict refs" in use at /usr/share/perl5/Bio/Tools/SeqStats.pm line 726.

#!/usr/bin/perl #use SeqStats; use Data::Dumper; use Bio::SeqIO ; use Bio::Seq ; use Bio::Tools::SeqStats; use Bio::Root::Root; # Calculate mean Kyte-Doolittle hydropathicity (aka "gravy" score) my $prot = Bio::PrimarySeq->new(-seq=>'MSFVLVAPDMLATAAADVVQIGSAVSAGS +',-format => 'Fasta',-alphabet=>'protein', -id=>'test'); $gravy = Bio::Tools::SeqStats->hydropathicity($seqobj); print "might be hydropathic" if $gravy > 1;