Here is a non-regex solution, too:
#!/usr/bin/env perl
use strict;
use warnings;
my %hash = map { chomp; split /\t/; } <DATA>;
use Data::Printer;
p %hash;
__DATA__
TCONS_00000047 XLOC_000039
TCONS_00000718 XLOC_000456
TCONS_00000938 XLOC_000610
TCONS_00004086 XLOC_002872
TCONS_00004252 XLOC_003003
TCONS_00004975 XLOC_003624
TCONS_00004976 XLOC_003624
TCONS_00005492 XLOC_004020
Output:
{
TCONS_00000047 "XLOC_000039",
TCONS_00000718 "XLOC_000456",
TCONS_00000938 "XLOC_000610",
TCONS_00004086 "XLOC_002872",
TCONS_00004252 "XLOC_003003",
TCONS_00004975 "XLOC_003624",
TCONS_00004976 "XLOC_003624",
TCONS_00005492 "XLOC_004020"
}
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