A few comments:
You never use $check. One thing I've taken on as a habit is to declare the variables I use as 'my' when I use them. When you pre-declare them in one string as you have with my ($ua, $req, $chrom, $start, $end, $db, $check);, you increase the chance of error when you remove/add them later, as you have in this script. One possible solution is as follows:
my $ua = LWP::UserAgent->new;
my $chrom = "7";
my $start = "117287204";
my $end = "117475903";
my $db = "hg11";
my $url = "http://genome.ucsc.edu/cgi-bin/hgText";
(I also like to indent for readability, but that's more a matter of personal style.)
You also are not properly separating your POST object. It should look something like:
# Indented again
my $request = POST $url,
['hgsid' => "6765675",
'g' => "htcGetDna2",
'c' => 'chr'.$chrom,
'l' => $start,
'r' => $end,
'db' => $db,
'hgc.dna.out1' => 'lc',
'boolshad.hgc.dna.rc' => '1',
'Submit' => 'Submit'
];
Note the use of the single quotes around the key, which was missing in your original script. Adding the above fixes allows your code to work as intended.
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