If the file is small, then the above answer by le is acceptable.

If you expect the file to be large (such as log file) and you know the approximate format of the file (e.g. the lines tend to have max width of 80 characters), then you can use seek(-81,2) and then use the above solution to get the last 1.5 lines.

You might want to check if it contains a newline, as otherwise you probably need to read more.
Here is a code snippet:

open (F,"the file");
seek(F,-81,2);
while(<F>) { $l=$_}
warn if $.<1;   # Make sure we read more than one line
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