my $open;


$mw-> Button (-text =>'Open', -command =>\&open_file)->place(-x=>240, -y=>35); 
$mw-> Button (-text =>'Get Statistics',-command =>[\&get_statistics, $open])->place(-x=>320, -y=>35); 







sub open_file {$open = $mw->getOpenFile(
                               -filetypes => $types_OPEN,
                              -defaultextension => '.sff');
                                      $te->Load( "$open");}
if (defined $open and -e $open){ 
        $te->Load( "$open");
        $fastaFile = $open;
}




sub get_statistics {my $stat = Statistics::Descriptive::Full->new();
my (%distrib);
my @bins = qw/18 19 20 21 22 23 24 25 26 27 28/;
my $fastaFile = $open;
   unless (defined $fastaFile){
        warn "No file name supplied.\n";
        return;
   }
   open my $FASTA, '<', $fastaFile or warn "Couldn't open file: $!\n";
my $junkFirstOne = <FASTA>;

while (<$FASTA>) {chomp;
    my ($def,@seqlines) = split /\n/, $_;
    my $seq = join '', @seqlines;
    $stat->add_data(length($seq));}


%distrib = $stat->frequency_distribution(\@bins);

print "Total reads:\t" . $stat->count() . "\n";
print "Total nt:\t" . $stat->sum() . "\n";
print "Mean length:\t" . $stat->mean() . "\n";
print "Median length:\t" . $stat->median() . "\n";
print "Mode length:\t" . $stat->mode() . "\n";
print "Max length:\t" . $stat->max() . "\n";
print "Min length:\t" . $stat->min() . "\n";
print "Length\t# Seqs\n";
foreach (sort {$a <=> $b} keys %distrib) {
    print "$_\t$distrib{$_}\n";
}};