sub Passref {
    my ( $define, $seq, $count, $outfh ) = @_;
    chomp($define);
    my $len   = length($$seq);
    my %count = ( Miss => 0+( $$seq =~ m/^YCWLPHQIMTNKSRVADEGF/ig ) );
    # YCWLPHQIMTNKSRVADEGF doesn't appear in test file
    my %ratio = ( Miss => 0+( $count{Miss} / $len ) );
    for my $letter (qw[ E D A V R S K N T M I Q H P L W C Y F ]) {
        $count{$letter} = ( $$seq =~ m/$letter/g );
        $ratio{$letter} = $count{$letter} / $len;
    }
    print {$outfh} join "\t", $define, $len,
      @ratio{qw[ E D A V R S K N T M I Q H P L W C Y F Miss  ]}, "\n";
}