Hi, i am stuck.
i have a file which contains various stretches of DNA in fasta format e.g
>12343
ACTAGCTAGCTGATGTCGTCCTACACAT
>4567465
ACTAGCGGCGTGCCTAGTACGTACTTCTTG
in which each record is separated by a newline.
I am trying to write code that will return all sequences containing more than 250 bases.
But i think i am getting confused with nested loops etc because although my program runs fine it still returns everything. can anyone help??
this is my code:
#! /usr/local/bin/perl -w
use strict;
open (INPUT, $ARGV[0]) or die "unable to open file";
my $sequence;
my $count =0;
my $base = ("A"|"C"|"G"|"T");
my @array;
my $ideal = 250;
while (<INPUT>)
{
$sequence = $_;
@array = ();
@array = split (/\n/, $sequence);
foreach $base ($sequence)
{
$count++;
if ($count > $ideal)
{
print "$base\n";
}
}
}
i am new to perl and would appreciate any help.
lolly
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